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Even while Gabriel was on the family line with her father, Linda was getting ready to call her new broker to give him a long list of buy orders. As she was put on hold for a few seconds, waiting for the broker to pick up, her mind flashed back to the final, critical meeting that triggered this momentous decision in her now-stellar investment career. Her adviser had been ill and was unavailable. So, on the occasion of a holiday dinner at the Johnston residence, she asked her father for his opinion. I m afraid of stocks, Dad. I still can t shake off the experience I had with stocks last time, and I ve done so well without them. Everything I see tells me it s time to go back into the market, but I also say to myself, my current program isn t broke why fix it Johnston warmed up when he realized his daughter was finally asking his opinion about investments or about anything, for that matter. How well have you done he asked. She fetched a file folder she had left on the China cabinet and pulled out a sheet printed from Excel. Down the left side were the investment categories, and across the top of the columns were headings such as investment, maturity, purchase price, current price, closed or open, gain/loss percent and gain/loss $. Down the right-hand side was a bolded number in the last column showing the total dollar gains for each investment category, plus another bolded number in the previous column showing the average percentage gains. Johnston leaned over, gently placing his hand on his daughter s right shoulder while squinting at the spreadsheet. I ll probably

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S 107, spans the bilayer only twice using exclusively the segments TM2 and TM3 (Fig 63) The differences in topology of the anti-holin and holin chains support a mode of action in which the lysis trigger is given by the relative disposition of the TM1 segment In this sense, the N-terminus bearing the MK dipeptide impedes the insertion into the bilayer of TM1 that remains adhered onto the lipid surface The loss of such dipeptide allows the insertion of TM1 and the access of the chain N-terminus to the periplasm In other words, the charge provided by a single K residue is enough to stall and impede the insertion of the transmembrane segment 42.

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External references, commonly called xrefs, enable you to view any drawing as a reference while in your current drawing. The external drawing is not part of your current drawing. The current drawing keeps track of the location and name of an external reference so that you can always reference it easily. As with blocks, you can snap to objects in the external reference, thereby using it as a reference for the drawing process. You can also change the visibility settings of the xref s layers. Xrefs have several advantages over blocks: n Xrefs keep your drawing smaller than blocks. The externally referenced drawing doesn t become part of your drawing. Your drawing maintains only a reference (name and location) to the other drawing. n You always have the most updated version of the xref. Each time that you open a drawing, a current copy of the xref loads. By contrast, you would need to reinsert a file inserted as a block to see the most updated version. n In a team project, several people can use the same drawing as an xref, each having access to the latest changes. n Xrefs can be attached and detached easily for maximum flexibility, loaded and unloaded to display or hide them, or overlaid for temporary use. If you re only using the xref for reference, you may detach it before plotting.

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S21 Anti-holin and Holin: The Exit of TM1 from the Membrane Switches on the Function Contrary to the S holin, whose function is designed to allow the passage from the cytoplasm to the periplasm of a folded endolysin, the S21 holin function is tailored to provide the membrane lesions needed for the release from the membrane of a signal anchor release containing endolysin, a membrane depolarization event Membrane depolarization requires not a major bilayer disruption but the exchange of ions As in the case of the S holin, S21 exists as two chains of 71 and 68 residues differing in the N-terminus MKS tripeptide sequence Sequence analysis predicts the existence of two transmembrane domains and an -helical hairpin fold with both N- and C-termini projected to the cytoplasm (intracellular).

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