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How can we be sure that the solution we have obtained for the three-by-three system presented in Question 18-1 is correct

The 5 end of the top (sense) strand is on the left. Top strand RNA sequence. Decoded RNA sequence in 5 to 3 direction gives protein sequence in N to C direction (Fig. 5-1). DNA is a double-stranded molecule in which the structure of the second strand can be deduced from the structure of the first strand. The second strand is complementary (A s in the first strand match T s in the

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bers are affected in this manner, and the percentage of B cells at all sites is signi cantly higher than it is in PM Engel and Arahata interpreted these differences as indicating that the effector response in DM is predominantly humoral, whereas in PM the response is composed of cytotoxic T cells, clones of which have been sensitized to a yet unde ned surface antigen on the muscle ber Diagnosis This is secure if there is a subacutely evolving proximal weakness, typical changes in the muscle biopsy, EMG abnormalities of myopathic type with scattered brillation potentials, and elevated serum levels of CK For DM, the nding of one of the typical dermatic features is of great value However, all these criteria have been satis ed in only half of our cases, even in those who are later shown to be responsive to corticosteroids (see later).

One must, therefore, often accept the diagnosis when most of the criteria are met Fever, pain beyond that attributable to arthritic involvement, or a greatly elevated sedimentation rate ( 100 mm/ h) suggest an alternative diagnosis The distinction from IBM, discussed further on, can be dif cult and depends to a large degree on the muscle biopsy As mentioned earlier, there has been considerable discussion regarding the diagnostic criteria for the in ammatory myopathies; some of these issues and reservations about the frequency with which the diagnoisis is made are articulated by Amato and Griggs The main point of controversy has been the proposal that true PM is rare and greatly overdiagnosed (see van der Muelen et al), a notion with which we do not fully agree.

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In contrast, in ammatory myopathy coexists with numerous systemic diseases as discussed, and some authors consider it to be a syndrome rather than a disease The problem of determining which patients should have an extensive evaluation for a systemic malignancy and for connective tissue disease is a dif cult one Opinions vary on this point but we have adopted the practice of careful inspection of the chest x-ray on all patients and of undertaking a more extensive evaluation in patients over age 55 or in smokers of any age The evaluation in the latter group includes chest and abdominal computed tomography scans, stool guaiac CA-125, CEA, etc In patients with recent weight loss, anorexia, or other symptoms suggestive of malignancy we have included upper and lower endoscopy and even occasionally resorted to a body positron emission tomography scan.

(a) Use a transfbrnration linearizethe nrodeland evaluate to thc paramcters. Display the data and the rnodelfit on a grapn. (b) Pcrforrn sanre thc evaluation in (a) bul usenonlinear as regrcsslon. 14.14 Givcn thc data

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